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Under the Clinical Laboratory Improvement Amendments (CLIA) regulations, HLA testing is considered


A) low complexity.
B) high complexity.
C) waived.
D) moderate complexity.

E) None of the above
F) A) and D)

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Which of the following is not acceptable for specimens to be used for HLA typing?


A) ACD anticoagulant
B) Refrigerator storage up to 14 days
C) Room temperature storage up to five days
D) EDTA anticoagulant
E) Heparin anticoagulant

F) None of the above
G) A) and D)

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The Ficoll Hypaque technique isolates mononuclear cells by


A) cell surface marker affinity.
B) cellular adherence.
C) cell membrane solubility.
D) cell density.

E) A) and B)
F) C) and D)

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A lymphocyte preparations in which < 95% of the cells have ingested trypan blue must be discarded and not used for HLA typing.

A) True
B) False

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Class II HLA antigens are best determined from


A) monocytes.
B) T lymphocytes.
C) B lymphocytes.
D) neutrophils.

E) A) and B)
F) None of the above

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